DEVELOPMENT OF AN AUTOMATED IMMUNOTURBIDIMETRIC FERRITIN ASSAY PDF

Such assays are suitable for the measurement of proteins at concentrations >5– 10 mg/L. .. Development of an automated immunoturbidimetric ferritin assay. May 3, Different laboratory methods are used to quantify ferritin . assays and automated equipment developments, and in routine laboratory assays and Seco M. Development of an automated immunoturbidimetric ferritin assay. J. Colloid. Interface Sci. (2): – 3. Borque, L., Rus, A., Bellod, L., and Seco, M.L. Development of an automated immunoturbidimetric ferritin assay.

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Diagnostic use of serum ferritin encompasses prelatent and latent iron deficiency as well as iron overloading. Clin Chem ; 45 Suppl 6: After elution, the released protein was measured using the BCA procedure. Download figure Open in new tab. C-reactive protein and bacterial infection in preterm infants.

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Imumnoturbidimetric approach to antibody immunopurification could be extended to obtain reagents useful for the measurement of several other proteins at low concentrations The immunopurified antibody showed a similar trend, having a covalently coupled adsorption isotherm according to that of the nonimmunopurified antibody in the range studied 0. We then selected 0.

The covalently coupled isotherms of IgG anti-CRP on carboxyl-modified polystyrene particles nm diameter are shown in Fig. Jadsamount of antibody adsorbed; Jinamount of antibody added.

Biological or Clinical Significance: Ferritin is an acute-phase respondent, and so individuals with conditions association with an ferrittin response may have elevated serum ferritin levels that are not directly related iron stores.

Lancet ; The detection limits for CRP, defined as the mean plus 3 SD of the zero signal saline solutionwere 0. Increased serum C-reactive protein levels by immunonephelometry in patients with rapidly destructive hip osteoarthritis. CRP is a prognostic risk factor for coronary heart disease in both patients with angina stable or not and in apparently healthy subjects 2and distinguishes rapidly destructive vs slowly progressive osteoarthritis 3.

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Development of an automated immunoturbidimetric ferritin assay.

Coverage was calculated by measuring the uncoupled IgG present in the supernatants of the first centrifugation, after filtering with a Nucleopore polycarbonate filter pore diameter, 0. Colloidal stability and immunoreactivity.

Nevertheless, these results can be influenced by the sensitivity of the methodology used for CRP quantification. The microparticle reagents were prepared according to a previously published method by the carbodiimide [1-ethyl 3-dimethylamino-propyl carbodiimide chloride] procedure It is also used to distinguish between hypoferric anemia and hypochromic anemia chronic infection and tumor anemias, sideroblastic anemia or thalassemia. A new method of measuring C-reactive protein, with a low limit of detection, suitable for risk assessment of coronary heart disease.

J Biomater Sci Polym Ed ; 3: Colloidal particles coated with polyclonal IgG usually are not very stable, mainly because of the high antibody coverage needed to obtain good sensitivities 3.

Production of C-reactive protein and risk of coronary events in stable and unstable angina. We describe a microparticle reagent that we use in an optimized turbidimetric procedure with immunopurified polyclonal antibodies against CRP. Ann Clin Biochem ; Among the many commercially available assays for CRP, the most common use fluid-phase or particle-enhanced turbidimetric or nephelometric procedures 7.

Clinical Chemistry Nov46 11. The effect of varying the particle size on a reagent with a constant charge of 0. For the immunopurified anti-CRP antibodies, we used a modified procedure consisting of two sequential covalent coupling steps.

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Development of an automated immunoturbidimetric ferritin assay.

Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency. Research Article Technical Brief. The microparticle reagent response of the different antibodies to increasing CRP concentrations can be seen in Fig. Jump to section Article Acknowledgments References.

Show more Technical Briefs. It allows lower antibody coverage on the microparticle reagent and provides highly sensitive and robust particle-enhanced turbidimetric immunoassay for serum CRP.

Clin Chem ; Ferritin is protein that has a central role in the storage of iron. Eur J Pediatr immunoturbjdimetric We chose this microparticle reagent, with a coverage of 0. We found that at any given working dilution of microparticles studied, the initial absorbance decreases with increasing wavelength and increases with microparticle diameter, according to the light-scattering theory. There were no interferences from bilirubin, hemoglobin or rheumatoid factors.

Variability in the measurement of C-reactive protein in healthy subjects: We monitored the reaction kinetics at pH values of 6.

Clin Chem ; 42 Suppl 6: View inline View popup.

Results obtained with the present procedure correlated well with those obtained by a nephelometric procedure and with those obtained by an RIA. The procedure has been automated using the Falcor analyzer. Test Directory Entire Site. J Ot Methods ; We have developed a new procedure for turbidimetric measurement of ferritin concentration in human serum, based on latex microparticle agglutination technology.