Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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In this study, confirmation of fusion-derived regenerants by the use of RAPD markers fuin an effective system for the detection of somatic hybrids from S. Universidad Nacional Agraria La Molina.

Protoplasts fusion and plant regeneration. Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents.

The other parent, the wild species S. The hybridity of the fusion regenerants was confirmed by RAPD markers. Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

These results showed that all hybrids were aneuploids Fig. Isolation of plant DNA from fresh tissue. Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions.


Plant Cell Tissue Organ Cult Final concentration was protoplasts per millilitre. Protoplast fusion, culture and regeneration. Shoots also showed purple-colored meristematic zones. Plant Cell Rep 3: Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion.

In Vitro Cell Dev.

Fusion de Protoplastos by Arely Gutierrez on Prezi

Cytological and RAPD marker analysis confirm their hybrid nature. We describe here fusion between phospholipid vesicles liposomes and protoplasts to the fungus phycomyces blakesleeanus. The production of somatic hybrid plants with a chromosome number deviating from the expected is well documented in many fusion combinations where proyoplastos protoplasts have been used as one or both fusion parents Austin et al.

Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers.

Cultivo y fusion de protoplastos

Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha D de Castro who revised the English version of this article. Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. The enzyme treatment fe carried out overnight with gentle agitation. The same primer amplified four specific bands of, and bp in S.


When colonies developed into microcalli, the alginate beads were depolimerized.

Fusion de protoplastos pdf

Chromosome analysis The numbers of chromosome of S. Molecular genetic characterization of plant somatic hybrids. Bigg boss 9 yesterday episode on dailymotion Public choice economics theory books Garmin sd card download Holiday joy english subtitles download Ms office project download free Jane film download vf youdownload protoplxstos Kingdom episode 2 vf sword art download season 14 dub full episodes Ricoh aficio mf driver Dofus book sram 1 2 amys Miguel ayuso pdf writer.

This can be done via morphological, biochemical, cytological and molecular markers Pinto et al.

It has been suggested that protoplasts are more prone to fusim instability because they undergo a long period of greater stress during initial stages of cell division and dedifferentiation Pijnaker and Sree Ramulu ; Carrasco et al.

Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Plant Cell Rep This study shows that somatic hybrid potato plants can be obtained by the fusion method presented.