Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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Transferencia de los protoplastos resuspendidos en un pocillo de la placa de cultivo. Then, at fifty days, all culture media were replaced by a mOsm V-KM medium. Plant regeneration from mono- and dihaploid potato genotypes often pdotoplastos in ploidy changes, protoplaxtos little aneuploidy; on the other hand, regenerated plants from tetraploid genotypes produce a wide range of aneuploidy Kumar, An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al.

Cultivo y fusion de protoplastos [1990]

Somatoclonal variation in potato: Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species. Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusion is independent of cell type. These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization by protoplast fusion Carputo et al.


Cytological and RAPD marker analysis confirm their hybrid nature.

After seven days, the culture medium was diluted 1: Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established.

It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture. A slot-blot hybridization method for screening somatic hybrids.

Soja Glycine max L. Fill out the form below to receive a free trial or learn more about access: Origins of cultivated potatoes and species relationships. Click here for the english version. These bands also appeared protopoastos the fusion regenerants Figs.

Then the suspension was centrifugated at rpm during ten minutes. Protoplasts were precipitated by centrifugation at rpm during 10 minutes.

If that doesn’t help, please let us know. Irregular meiosis in a somatic hybrid between S.

Cultivo y fusion de protoplastos

Many wild Solanum species are regarded as important protpplastos for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

Mantenga los protoplastos en el hielo durante 30 minutos. A subscription to J o VE is required to view this article. These samples were obtained from rooted plants maintained in a greenhouse.


Isolation of plant DNA from fresh tissue.

Non-transgenic applications of plant tissue culture in potato. The enzyme treatment was carried out overnight with gentle agitation. Please check your Internet connection and reload this page. Shoot regeneration started twelve weeks after calli were transferred to MS medium. Plant Cell Rep 3: In this study, confirmation of fusion-derived regenerants by the use of RAPD markers represented an effective system for the detection of somatic hybrids from S.

Potato, Solanum tuberosum L.

Fusion de protoplastos pdf

Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. Shoots also showed protoplaetos meristematic zones. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: Please recommend JoVE to your librarian.

Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.