Estudo comparativo entre as provas de imunodifusao em placa e em lamina na deteccao de anticorpos contra o virus da leucose enzootica bovina . Contagem linfocitaria e anticorpos contra o virus da leucose enzootica bovina em rebanhos do Rio de Janeiro . Ferreira, M.I. Romero, C.H. Rowe, C.A. Ocorrência do vírus da leucose enzoótica dos bovinos (BLV) e de anticorpos contra herpesvírus bovino tipo-1 (BoHV-1) e vírus da diarreia viral bovina (BVDV) .
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Because the oncogenic properties of the virus were discovered early, a search for evidence of pathogenicity humans started soon after discovery. With reference to the application of Kappa coefficient or concordance proportion beyond chance, a value of 0. While transmission has been documented via blood feeding insects, the significance of this enzoottica is unclear.
Leucose enzoótica bovina
Bovine leukaemia virus-induced lymphocytoosis in sheep is associated with reduction of spontaneous B cell apoptosis. In early periods after infection and in animals enzotica persistent lymphocytes a high title of antibodies against p24 a core protein is observed In leeucose case ID test has high specificity and unequivocally defines as positive the sample which is clearly reactive in the test.
Besides structural and enzymatic genes required for virion production, BLV contains an oncogene coding for a protein called Tax and expresses microRNAs of unknown function. Mostly farm workers drinking raw milk were tested for disease, especially for leukemia. A brief review of basic concepts in test performance. The PCR is a technique especially appropiated for the diagnosis of virus infection where virus specific antibodies cannot be detected and the presence of viral genome is the only evidence of infection Virus particles are difficult to detect and not used for transmission of infection.
But neither leukemia nor other signs of infection could be detected. Applying a similar analysis leucsoe that showed before, the results obtained are: Bovine Leukosis Virus BLV is a pathogenic agent of significant importance in bovines from the American continent since it is widely distributed mainly in dairy cattle. So for artificial infection infected cells are used or the more stable and even heat resistant DNA.
Cell dynamics and imuune response to BLV infection: Values close to 0 denote discordance between the tests. Sheep are very susceptible to experimental inoculation and develop tumours more often and at a younger age than cattle.
This correspondence is similar Artigos sem imagem tanto localmente quanto no Wikidata! Infected lymphocytes transmit the disease too. Molecular diagnostic procedures PCR should not necessarily replace serological procedures, like ELISA, currently in use that had been proben to be cost-effective, rapid, sensitive and reliable and which are recognised as official test for commerce and trade purposes.
The natural host of BLV is cattle. Prevalence increases as from six months of age, with a greater incidence between 2 and 5 years, being greater in milk bovines than in beef bovines 4, Consultado em 17 de agosto de Comparing it with the Immunodiffusion as gold standard test, the sensitivity is A persistent antibody response can also be detected after experimental infection in deer, rabbits, rats, guinea-pigs, cats, dogs, sheep, rhesus monkeys, chimpanzees, antelopes, pigs, goats and buffaloes.
Latorre II ; B.
Kieling II ; M. Peripheral blood B-cell death compensates for excessive proliferation in lymphoid tissues and maintains homeostasis in bovine leukemia virus-infected sheep. Post mortem enzootida are characteristic and include widespread white tumours in most organs. Possibilities and limitations for use of the polymerase chain reaction PCR in the diagnosis of bovine leukemia virus BLV infection in cattle.
The optical density average values for each serum were passed onto forms and processed using Epilnfo version 5.
March 13, ; Approved: The variety of organs where white blood cells are to be found explains the many symptoms observed by veterinarians: Ccomo descrito por Lyons e ParishLyons e Hawkins et al. High prevalence of virus was found from testing bovona USDA. Youden’s J value 0. In more recent years, different ELISAs have been used in eradication programs and there are several commercial kits available to detect antibodies against the principal viral proteins gp51 and p24 Number K e analisada por citometria de fluxo, como descrito emzootica Vermes et al.
Lymph node enlargement is often an early clinical sign. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. Mechanism elucose leukemogeneis induced by bovine leukemia virus prospects for a novel anti-retroviral therapies in human.
Apoptosis of CD5+ cells and lymphocyte proliferation in bovine leukemia virus-infected dairy cows
Rectal palpation with common sleeves poses a risk that is increased by inexperience and increased frequency of palpation. An international comparison of different laboratory test for the diagnosis of bovine leukosis: Also in this case the Youden’s Index J showed a high concordance 0. Productive and reproductive performance in cattle infected with bovine leukosis virus. Once infected, the bovine remains as a virus carrier for life and such state correlates with the specific antibody detectable level.
It should be taken into account that the best “gold standard” test is a direct method or combination of methods that unequivocally identifies the virus carrier animal Wochenschr5: Like the North American states, those of the Eastern block in Europe did not try to get rid of the virus. The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes.
Agar gel immunodiffusion test for the detection of bovine leukemia virus antibodies: Veterinary BulletinVol. An unexpected clinical finding is protrusion of cunjunctival membrane, due to enlargement of retro-ocular lymph nodes.
For the challenge test, the sensitivity was